The victims, 47-year-old Marion Anderson and 59-year-old Lizette Sorrano, were struck on Forest Hill Road near Pilcher Street at around 6:30 p.m. on Wednesday.
The accident happened just outside the entrance to the Staten Island Developmental Disabilities Services Office, where the women were coworkers.
The vehicle that hit them, a mini-van, remained at the scene. Police say no criminality is suspected in the accident.
The victims were taken to Staten Island University Hospital in Ocean Breeze where they were pronounced dead.