The crash occurred around 4:30 a.m. Tuesday at Foster and Ralph avenues in the East Flatbush section.
The NYPD says the 54-year-old victim collided with a vehicle driven by a 22-year-old man. He was in stable condition at a hospital.
Police say that after the initial crash, the woman's car collided with the tractor trailer as it approached the intersection. The truck driver was not hurt.
The victim's name was not immediately released pending family notification.