Trustee Irving H. Picard says 22 lawsuits were filed against relatives of both Madoff and his wife, Ruth.
Her attorney didn't immediately respond to an e-mailed request for comment Friday night.
Eighteen lawsuits were filed against former employees of Bernard L. Madoff Investment Securities LLC.
Picard says his firm is seeking about $69 million in funds deposited by the company's customers and stolen in the 72-year-old's vast Ponzi scheme.
Madoff is serving a 150-year sentence after confessing to the decades-long scheme.
An estimated $20 billion was lost, making it the biggest investment fraud in U.S. history.